Short-term high NaCl dietary intake changes leukocyte expression of VLA-4, LFA-1 and Mac-1 integrins in both healthy humans and Sprague- Dawley rats ; a comparative study (CROSBI ID 267978)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Mihalj, Martina ; Matić, Anita ; Mihaljević, Zrinka ; Barić, Lidija ; Stupin, Ana ; Drenjančević, Ines
engleski
Short-term high NaCl dietary intake changes leukocyte expression of VLA-4, LFA-1 and Mac-1 integrins in both healthy humans and Sprague- Dawley rats ; a comparative study
This study aimed to assess the effects of short-term high salt (HS) diet on the peripheral blood leukocytes (PBL) activation status in healthy rats and young human individuals. Distribution of PBL subpopulations and surface expression of integrins were determined using flow cytometry in 36 men and women on 7-days low-salt diet (<3.2g salt/day) immediately followed by 7-days HS diet (~14g salt/day), or in Sprague-Dawley (SD) rats (N=24) on 0.4% NaCl diet (aLS group) or 4% NaCl diet (aHS group) for 7 days. aHS group presented with increased frequency of granulocytes, while the frequency of lymphocytes was reduced. Although in humans HS diet reduced the expression of CD11b(act) integrin on lymphocytes, the frequency of CD11b(act)-bearing cells among all PBL subsets was increased. aHS group of rats exhibited increased expression of total CD11b/c in granulocytes and CD3 lymphocytes. Expression of CD11a was significantly reduced in all PBL subsets from human subjects and increased in aHS group. CD49d expression on all PBL subsets was significantly decreased in both human and rats. In human subjects, we found reduced frequencies of intermediate monocytes accompanied by reciprocal increase in classical monocytes. Present results suggest that short- term HS diet can alter leukocytes’ activation status and promote vascular low-grade inflammation.
high salt diet ; Sprague-Dawley rats ; humans ; LFA-1 ; VLA-4 ; Mac-1 integrins ; peripheral blood leukocytes
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Podaci o izdanju
2019 (/)
2019.
6715275
18
objavljeno
0962-9351
1466-1861
10.1155/2019/6715275
Trošak objave rada u otvorenom pristupu
Povezanost rada
Temeljne medicinske znanosti