engleski

Designing ColorX, Image Processing Software for Colorimetric Determination of Concentration, To Facilitate Students’ Investigation of Analytical Chemistry Concepts Using Digital Imaging Technology

This work combines the laboratory quantitative analysis of colored solutions and common devices for digital imaging (digital or web camera or mobile phones, i.e., smartphones). ColorX software, specially designed for this study, was used for data collection and analysis in order to calculate concentration of colored solutions from measured RGB values. Three different custom methods for determination of concentration have been developed: (i) RGB value measurement by pixel ; (ii) RGB value measurement by pixel and Gaussian blur ; and (iii) calculation of average RGB value of the selected image area. The performance of the developed software ColorX is demonstrated using different colored solutions: KMnO4 (purple) ; CoSO4 (red) ; NiSO4 (green) ; and CuSO4 (blue) solutions, as well as the Lowry protein assay (blue) to determine the concentration of an unknown sample. The most suitable and effective method for study of the mentioned solutions was calculation of average RGB value of the selected image area. ColorX software is primarily designed for accessibility and simplicity with the aim of promoting and encouraging students to explore and discover potential applications of digital imaging technology in basic analytical chemistry concepts.

High School ; Introductory Chemistry ; Analytical Chemistry ; Computer-Based Learning ; Laboratory Equipment ; Apparatus ; Quantitative Analysis

nije evidentirano