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Pregled bibliografske jedinice broj: 1013453

Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart


Polančec, Denis; Zenić, Lucija; Hudetz, Damir; Borić, Igor; Jeleč, Željko; Rod, Eduard; Vrdoljak, Trpimir; Skelin, Andrea; Plečko, Mihovil; Turkalj, Mirjana et al.
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart // Genes, 10 (2019), 474, 11 doi:10.3390/genes10060474 (međunarodna recenzija, članak, znanstveni)


Naslov
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Autori
Polančec, Denis ; Zenić, Lucija ; Hudetz, Damir ; Borić, Igor ; Jeleč, Željko ; Rod, Eduard ; Vrdoljak, Trpimir ; Skelin, Andrea ; Plečko, Mihovil ; Turkalj, Mirjana ; Nogalo, Boro ; Primorac, Dragan

Izvornik
Genes (2073-4425) 10 (2019); 474, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lipoaspirate ; microfragmentedlipoaspirate ; immunophenotyping ; endothelialprogenitors ; pericytes ; supra-adventitialadiposestromalcells

Sažetak
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF- LA and SVF-MLA samples, the following population phenotypes were identified within the CD45– fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34– CD73±CD90±CD105–CD146± mature endothelial cells, CD31–CD34–CD73±CD90+CD105–CD146+ pericytes, CD31–CD34+CD73±CD90+CD105–CD146+ transitional pericytes, and CD31–CD34+CD73highCD90+CD105– CD146– supra-adventitial-adipose stromalcells(SA-ASC). Theimmunophenotyping profile ofSVF-MLAwas dominatedby areduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte- primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

Izvorni jezik
Engleski

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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