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Pregled bibliografske jedinice broj: 1013453

Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart


Polančec, Denis; Zenić, Lucija; Hudetz, Damir; Borić, Igor; Jeleč, Željko; Rod, Eduard; Vrdoljak, Trpimir; Skelin, Andrea; Plečko, Mihovil; Turkalj, Mirjana et al.
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart // Genes, 10 (2019), 6; 474, 11 doi:10.3390/genes10060474 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1013453 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Autori
Polančec, Denis ; Zenić, Lucija ; Hudetz, Damir ; Borić, Igor ; Jeleč, Željko ; Rod, Eduard ; Vrdoljak, Trpimir ; Skelin, Andrea ; Plečko, Mihovil ; Turkalj, Mirjana ; Nogalo, Boro ; Primorac, Dragan

Izvornik
Genes (2073-4425) 10 (2019), 6; 474, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
lipoaspirate ; microfragmentedlipoaspirate ; immunophenotyping ; endothelialprogenitors ; pericytes ; supra-adventitialadiposestromalcells

Sažetak
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF- LA and SVF-MLA samples, the following population phenotypes were identified within the CD45– fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34– CD73±CD90±CD105– CD146± mature endothelial cells, CD31–CD34– CD73±CD90+CD105–CD146+ pericytes, CD31– CD34+CD73±CD90+CD105–CD146+ transitional pericytes, and CD31–CD34+CD73highCD90+CD105– CD146– supra-adventitial-adipose stromalcells(SA-ASC). Theimmunophenotyping profile ofSVF-MLAwas dominatedby areduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte- primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

Izvorni jezik
Engleski



POVEZANOST RADA


Ustanove
Medicinski fakultet, Rijeka,
Medicinski fakultet, Zagreb,
Klinička bolnica "Sveti Duh",
Medicinski fakultet, Split,
Medicinski fakultet, Osijek,
Sveučilište u Rijeci - Odjel za biotehnologiju,
Fakultet za dentalnu medicinu i zdravstvo, Osijek

Citiraj ovu publikaciju

Polančec, Denis; Zenić, Lucija; Hudetz, Damir; Borić, Igor; Jeleč, Željko; Rod, Eduard; Vrdoljak, Trpimir; Skelin, Andrea; Plečko, Mihovil; Turkalj, Mirjana et al.
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart // Genes, 10 (2019), 6; 474, 11 doi:10.3390/genes10060474 (međunarodna recenzija, članak, znanstveni)
Polančec, D., Zenić, L., Hudetz, D., Borić, I., Jeleč, Ž., Rod, E., Vrdoljak, T., Skelin, A., Plečko, M. & Turkalj, M. (2019) Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes, 10 (6), 474, 11 doi:10.3390/genes10060474.
@article{article, year = {2019}, pages = {11}, DOI = {10.3390/genes10060474}, chapter = {474}, keywords = {lipoaspirate, microfragmentedlipoaspirate, immunophenotyping, endothelialprogenitors, pericytes, supra-adventitialadiposestromalcells}, journal = {Genes}, doi = {10.3390/genes10060474}, volume = {10}, number = {6}, issn = {2073-4425}, title = {Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart}, keyword = {lipoaspirate, microfragmentedlipoaspirate, immunophenotyping, endothelialprogenitors, pericytes, supra-adventitialadiposestromalcells}, chapternumber = {474} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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  • BIOSIS Previews (Biological Abstracts)


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