engleski

Anal sac apocrine adenocarcinoma of dogs-a histological, immunohistochemical and clinical characterization

Anal sac apocrine adenocarcinoma (ASAA) is the most common malignant tumor of the perianal region of dogs. Its malignancy is reflected in common metastases to distant sites and usually apparent paraneoplastic syndrome. In this study, incidence, biological behavior, histologic features and proliferation index of anal sac apocrine gland adenocarcinoma are investigated. The study included 17 samples of ASAA which were retrieved from the archives of Department of Veterinary pathology, Veterinary faculty, University of Zagreb, years 2008-2016. All of the samples were thoroughly microscopically examined. The samples were also analysed using immunohistochemical Ki-67 marker (MIB-1, Dako). Finally, a data search which included the survey of the owners was conducted to gain clinical data on biologic behaviour of each particular tumor. The results showed greater incidence of ASAA in golden and Labrador retrievers (seven tumors, 41, 2% ). Average age of dogs was 9.22 years, 12 samples (70.6%) were from male dogs, and 5 (29.4%) from female dogs. There were 12 samples of tumors of a solid subtype and 5 of a tubular subtype. Tumor recurrence was noted in 55.5 % of dogs, and the metastases in 44.4% of dogs. The average value of Ki-67 expression in all of the samples was 24, 5% (25.7% in a solid subtype of tumors, and 21.7% in the tubular subtype). The average value of Ki-67 expression in the group of small tumors (diameter less than 2cm) was 18.65%, while in the group of large tumors (diameter of tumor equal or more than 2cm) it was 32.88% - this difference is also statistically significant (p= 0, 0112). In conclusion, larger tumors had a significantly higher expression of Ki-67, which could point to their later phase in the process of carcinogenesis. We believe that a larger number of samples would also show significant differences in the expression of Ki-67 according to the different histological tumor subtype.

anal sac apocrine adenocarcinoma ; histology ; immunohistochemistry ; dog

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