Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1012090

Characterisation of the integrin αv adhesome in human melanoma cells RPMI-­7951


Dekanić, Ana; Paradžik, Mladen; Humphries, J.D.; Nestić, Davor; Majhen, Dragomira; Humphries, Martin; Ambriović ­Ristov, Andreja
Characterisation of the integrin αv adhesome in human melanoma cells RPMI-­7951 // YSF FEBS 2019 Abstract book / FEBS 2019 Congress Secreteriat (ur.).
Krakov: Polish Biochemical Society, 2019. str. 92-92 (predavanje, nije recenziran, sažetak, ostalo)


CROSBI ID: 1012090 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Characterisation of the integrin αv adhesome in human melanoma cells RPMI-­7951

Autori
Dekanić, Ana ; Paradžik, Mladen ; Humphries, J.D. ; Nestić, Davor ; Majhen, Dragomira ; Humphries, Martin ; Ambriović ­Ristov, Andreja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
YSF FEBS 2019 Abstract book / FEBS 2019 Congress Secreteriat - Krakov : Polish Biochemical Society, 2019, 92-92

Skup
The 19th FEBS Young Scientists' Forum

Mjesto i datum
Krakov, Poljska, 03-06.07.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
integrin alphav ; adhesome ; integrin adhesion complex

Sažetak
Integrins are transmembrane receptors often overexpressed in melanoma that can modulate response to antitumour therapy. They transmit signals from extracellular matrix (ECM) to cytoskeleton through a multimolecular complex of signalling and adaptor proteins called integrin adhesion complex (IAC). The total sum of proteins of the IAC (adhesome) represents a pool of insufficiently investigated potential targets for tumour therapy. Our previous results on human melanoma cells RPMI­7951 have shown that targeted knockdown of integrin subunit αv via siRNA increased sensitivity to antitumour drugs: cisplatin, paclitaxel or vincristine, and drastically decreased in vitro migration and invasion. The goal of this study was: a) to define adhesome of RPMI­7951 cells that have assembled their own ECM, b) to determine key integrins these cells use to form IACs and c) to define IAC components dependent on integrin αv in order to pinpoint proteins involved in the observed phenotype of increased sensitivity to drugs and decreased motility. IACs were isolated from RPMI­7951 cells transfected with control or integrin αv­ specific siRNA plated on uncoated Petri dishes, upon crosslinking with DTBP and analysed by mass spectrometry. We identified 344 proteins, with a minimum number of spectra 4, in at least one of the three replicas. 31.9% of proteins are focal adhesion­ and 25.5% ECM­ associated proteins. Data suggest that RPMI­7951 preferentially form IACs via integrin αvβ5. 88 proteins, whose expression was changed at least 2 times in RPMI­7951 cells after integrin αv knockdown, were defined as integrin αv adhesome. These proteins might have a role in the observed phenotype of increased sensitivity to antitumour drugs and reduced motility. Further studies of IAC proteins could lead to the identification of potential target molecules for combined use with antitumour drugs for the metastatic melanoma treatment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-2465 - Molekularni mehanizmi povećanja osjetljivosti na protutumorske lijekove stanica karcinoma dojke i melanoma čovjeka utišavanjem integrina (INSILCELL) (Ambriović Ristov, Andreja, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju:

Dekanić, Ana; Paradžik, Mladen; Humphries, J.D.; Nestić, Davor; Majhen, Dragomira; Humphries, Martin; Ambriović ­Ristov, Andreja
Characterisation of the integrin αv adhesome in human melanoma cells RPMI-­7951 // YSF FEBS 2019 Abstract book / FEBS 2019 Congress Secreteriat (ur.).
Krakov: Polish Biochemical Society, 2019. str. 92-92 (predavanje, nije recenziran, sažetak, ostalo)
Dekanić, A., Paradžik, M., Humphries, J., Nestić, D., Majhen, D., Humphries, M. & Ambriović ­Ristov, A. (2019) Characterisation of the integrin αv adhesome in human melanoma cells RPMI-­7951. U: FEBS 2019 Congress Secreteriat (ur.)YSF FEBS 2019 Abstract book.
@article{article, author = {Dekani\'{c}, Ana and Parad\v{z}ik, Mladen and Humphries, J.D. and Nesti\'{c}, Davor and Majhen, Dragomira and Humphries, Martin and Ambriovi\'{c} \-Ristov, Andreja}, year = {2019}, pages = {92-92}, keywords = {integrin alphav, adhesome, integrin adhesion complex}, title = {Characterisation of the integrin αv adhesome in human melanoma cells RPMI-\-7951}, keyword = {integrin alphav, adhesome, integrin adhesion complex}, publisher = {Polish Biochemical Society}, publisherplace = {Krakov, Poljska} }
@article{article, author = {Dekani\'{c}, Ana and Parad\v{z}ik, Mladen and Humphries, J.D. and Nesti\'{c}, Davor and Majhen, Dragomira and Humphries, Martin and Ambriovi\'{c} \-Ristov, Andreja}, year = {2019}, pages = {92-92}, keywords = {integrin alphav, adhesome, integrin adhesion complex}, title = {Characterisation of the integrin αv adhesome in human melanoma cells RPMI-\-7951}, keyword = {integrin alphav, adhesome, integrin adhesion complex}, publisher = {Polish Biochemical Society}, publisherplace = {Krakov, Poljska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font