engleski

Acute promyelocytic leukemia M3: cytomorphologic, immunophnenotypic, cytogenetic, and molecular variants

Acute promyelocytic leukemia M3 is AML subtype characterized by proliferation of malignant promyelocytes with mature myeloid immunophenotype and atypical reciprocal translocation involving chromosome 15 and 17 /t(15 ; 17)(q22 ; q11)/ resulting in the fusion of retinoic acid receptor alpha (RAR alpha) on chromosome 17 and the putative factor PML. There are three M3 morphologic variants: typical hypergranular form, hypogranular and basophilic variant. However, some of patients have not specific translocation, or they have other cytogenetic abnormalities beside or without characteristic ones. The aim of the study was to compare morphologic, immunologic, cytogenetic and molecular characteristic of blasts in 15 patients with M3 treated in Clinical Hospital Zagreb, Zagreb, Croatia. In major M3 patients (13) blasts had typical hypergranular appearance with mature myeloid immunophenotype (HLA-DR, CD13+, CD33+). Typical translocation /t(15 ; 17)(q22 ; q11)/ was detected by cytogenetic analysis in 5 M3 patients but PML/RAR was positive in 11 out of 14 patients with RT-PCR. In one patient t(17 ; 17) was found, in 2 patients +8 one patient had also add (7)(q22) and -7. Patients with complete remission rate (13) became PML/RAR negative. Four of them reverted to PCR positivity and relapsed within 3 months.

acute promyelocytic leukemia ; M3 ; t(15 ; 17)(q22 ; q11) ; immunophenotype

Rad je kao poster, na hrvatskom jeziku, prezentiran i na skupu II. hrvatski kongres hematologa i transfuziologa s međunarodnim sudjelovanjem, održanom od 16-19.10.1999., Dubrovnik, Hrvatska ; sažetak je objavljen u Liječnički vjesnik. Supplement 121 (1999) str. 154-154 ; a na engleskom jeziku na skupu 17th International Meeting and 13th European Congress of Clinical Chemistry and Laboratory Medicine, održanom od 06.-11.06.1999., Firenza, Italija ; sažetak objavljen u 'Clinical chemistry and laboratory medicine' 2 (1999) (S) str. 216-216.