Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen (CROSBI ID 267152)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Štambuk, Nikola ; Konjevoda, Paško ; Turčić, Petra ; Šošić Hrvoje ; Aralica, Gorana ; Babić, Damir ; Seiwerth, Sven ; Kaštelan, Željko ; Novak Kujundžić, Renata ; Wardega, Piotr ; Barać Žutelija, Jelena ; Gudelj Gračanin, Ana ; Gabričević, Mario
engleski
Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen
The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense–antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines.
neoplasm ; prostate specific antigen ; biomarker ; antisense peptide ; immunohistochemistry ; nanomedicine
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Podaci o izdanju
20 (9)
2019.
2090
20
objavljeno
1422-0067
10.3390/ijms20092090
Povezanost rada
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Kliničke medicinske znanosti