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Headspace screening of alga Colpomenia sinuosa (Mertens ex Roth) Derbès & Solier, 1851 volatiles

Colpomenia sinuosa (Mertens ex Roth) Derbès & Solier, 1851 is thallus bladder-like, smooth, slick, hollow, crisp, spherical to sac-like, irregularly expanded or somewhat lobed, to 30 cm diameter, 10 cm high. This golden-brown alga belongs to Scytosiphonaceae family. The sample was collected in the Adriatic sea close to Island Vis (43° 03' 47'' N, 16° 11' 15'' E). Volatiles of fresh C. sinuosa were extracted by headspace solid-phase microextraction (HS-SPME) and analysed by gas chromatography and mass spectrometry (GC-MS). In this investigation of alga headspace volatile organic compounds (VOCs) two fibers were used: polydimethylsiloxane/divinylbenzene (PDMS/DVB) and divinylbenzene/carboxene/polydimethylsiloxane (DVB/CAR/PDMS). The major C. sinuosa volatile compounds (PDMS/DVB ; DVB/CAR/PDMS) were: dictyopterene D (6-[(Z)-1-butenyl]-1, 4-cycloheptadiene) (26, 7 % ; 10, 9 %), Dictyopterene C' (6-Butyl-1, 4-cycloheptadiene) (11, 4%, 2, 7 %), dimethyl sulfid (8, 4% ; 18, 3 %), tridecanal (5, 1% ; 6, 3%), pentadecanal (5, 0% ; 2, 4%), 1-pentene-3-on (2, 2% ; 3, 4%), octan-1-ol (1, 2% ; 3, 1%), B-Ionone (3, 8% ; 5, 8%) and S-methyl thioacetate (- ; 3, 3 %). Application of headspace solid-phase microextraction enables identification of low-molecular organic compounds. Among those volatiles biologically active compound can be found, such as allelochemicals, defensive compounds, sex attractants or pheromones.

Colpomenia sinuosa, gas chromatography-mass spectrometry, headspace solid-phase microextraction, volatile organic compounds

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