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Pregled bibliografske jedinice broj: 1009109

Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity


Baković, Petra; Kesić, Maja; Lucien Jean Proust, Bastien; Štefulj, Jasminka; Čičin-Šain, Lipa
Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity // Pokusne životinje u znanstvenim istraživanjima 2018, 3. znanstveno-stručni simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama, 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem
Zagreb, Hrvatska, 2018. str. 34-34 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity

Autori
Baković, Petra ; Kesić, Maja ; Lucien Jean Proust, Bastien ; Štefulj, Jasminka ; Čičin-Šain, Lipa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pokusne životinje u znanstvenim istraživanjima 2018, 3. znanstveno-stručni simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama, 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem / - , 2018, 34-34

Skup
3. znanstveno-stručni simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama, 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 25.-26.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Wistar-Zagreb 5HT rat, serotonin, high-fat diet, obesity, animal model

Sažetak
The continuously increasing prevalence of obesity worldwide urges development of animal models sharing features of human obesity, that would enable a search for more effective preventive and/or treatment strategies. Currently available rodent models of obesity are based on genetic manipulations of one or few target genes or on exposures to obesogenic environment such as high-fat diet. Serotonin (5HT), an ancient biogenic amine, plays a vital role in energy homeostasis and has been implicated in the pathogenesis of obesity. Here we will present a potentially novel animal model in obesity research, the two sublines of the Wistar-Zagreb (WZ) 5HT rats, developed by selective breeding toward physiological extremes of platelet serotonin parameters. Selective breeding procedure resulted in high- 5HT and low-5HT subline of rats with constitutionally upregulated and downregulated, respectively, 5HT tone. High-5HT animals have a lifelong increased body weight and elevated adiposity as compared to low-5HT rats. On the other hand, low-5HT rats seem to be more sensitive to obesogenic effects of high-fat diet. Our animals are kept 3 per cage with free access to commercial rat chow (Mucedola, 4RF21 or MD.06415, 45% kcal from fat) and tap water, and are housed under controlled conditions (23±2°C, 55±10% humidity, 12h light/12h dark). All experiments are conducted in accordance with national legislations (NN 55/2013 ; 102/2017) and the EU directive on the protection of animals used in scientific purposes (2010/63/EU). 3R principle and the ARRIVE guidelines are followed as much as possible and healthy status of animals is tested four times a year (Faculty of Veterinary Medicine, University of Zagreb).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2014-09-7827 - Serotonergična modulacija pretilosti: međuovisnost regulatornih molekula i puteva (Lipa Čičin-Šain, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Baković, Petra; Kesić, Maja; Lucien Jean Proust, Bastien; Štefulj, Jasminka; Čičin-Šain, Lipa
Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity // Pokusne životinje u znanstvenim istraživanjima 2018, 3. znanstveno-stručni simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama, 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem
Zagreb, Hrvatska, 2018. str. 34-34 (poster, domaća recenzija, sažetak, znanstveni)
Baković, P., Kesić, M., Lucien Jean Proust, B., Štefulj, J. & Čičin-Šain, L. (2018) Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity. U: Pokusne životinje u znanstvenim istraživanjima 2018, 3. znanstveno-stručni simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama, 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem.
@article{article, year = {2018}, pages = {34-34}, keywords = {Wistar-Zagreb 5HT rat, serotonin, high-fat diet, obesity, animal model}, title = {Wistar-Zagreb serotonin (WZ-5HT) rats - a new animal model of obesity}, keyword = {Wistar-Zagreb 5HT rat, serotonin, high-fat diet, obesity, animal model}, publisherplace = {Zagreb, Hrvatska} }