engleski

Effects of the antiepileptic valproate and of the flavonone naringenin on the antioxidative defence system in the brain of C57Bl6 mouse

Valproate is a widely used antiepileptic drug. It is considered generally safe, but adverse effects, mainly in lipid metabolism are known to occur among patients. Similarly, adverse effects caused by valproate in the antioxidative defence system, in various tissues, including the brain, are known from in vivo and in vitro experimental systems. Our aim was to investigate, whether the use of polyphenylene naringenine, a known antioxidant and antilipidemic agent, together with valproate, affects the redox balance, and whether the synergistic application of naringenine and valproate, can reduce the potential negative effects in C57Bl6 mice. Lipid peroxidation, the concentration of total glutathione and the antioxidant activity of superoxide dismutase were measured in the prefrontal and central cortex, and in the cerebellum, after 15 days of exposure to PBS (control), Valproate 400 mg/kg bw, naringenin (25 mg/kg be) and joint valproate+naringenine treatment (in the same doses as individual groups). Our results showed, that the treatment with valproate and naringenine, and their combinations, caused increased lipid peroxidation in both males and females, in all three measured brain regions. The activity of the superoxide dismutase, and of the reduced glutathione in the brain, has been differentialy activated dependent on the tratment. The protective effect of the polyphenolic naringenine molecule, has not been demonstrated as expected.

valproate, naringenin, brain, antioxidative effects

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