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Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment (CROSBI ID 266206)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Kostanjevečki, Petra ; Petrić, Ines ; Lončar, Jovica ; Smital, Tvrtko ; Ahel, Marijan ; Terzić, Senka Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment // Science of the total environment, 687 (2019), 858-866. doi: 10.1016/j.scitotenv.2019.06.118

Podaci o odgovornosti

Kostanjevečki, Petra ; Petrić, Ines ; Lončar, Jovica ; Smital, Tvrtko ; Ahel, Marijan ; Terzić, Senka

engleski

Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment

The biodegradation of biorecalcitrant opioid drug tramadol (TRAM) was studied in a model biodegradation experiment performed with an enriched activated sludge culture pre-adapted to high concentration of TRAM (20mg/L). TRAMand its transformation products (TPs)were determined by applying ultrahigh- performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS), the sludge culture was characterized using a 16S rRNA gene amplicon sequencing, whereas ecotoxicological evaluation was performed based on determination of toxicity to freshwater algae. Tramadol removal was much faster (t1/2=1.3 days) and more efficient in glucose-containing mineral medium (cometabolic conditions) than in a medium without glucose. The elimination of the parent compound resulted in the formation of five TPs, two of which (TP 249 and TP 235) were identified as N- desmethyltramadol (N-DM TRAM) and N, N- didesmethyltramadol (N, N- diDM TRAM). The remaining 3 TPs (TP 277a-c) were isomeric compounds with an elemental composition of protonated molecules C16H24NO3 and a putative structure which involved oxidativemodification of the dimethylamino group. Pronounced changes in the taxonomic composition of the activated sludge were observed during the enrichment, especially regarding an enhanced percentage of 8 genera (Bacillus, Mycobacterium, Enterobacter, Methylobacillus, Pedobacter, Xanthobacter, Leadbetterella and Kaistia), which might be related to the observed transformations. The removal of TRAMresulted in proportional reduction of algal toxicity, implying a positive result of the accomplished transformation processes.

Tramadol ; Biotransformation ; Taxonomic characterization ; Sludge culture ; Transformation products ; Ecotoxicological assessment

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Podaci o izdanju

687

2019.

858-866

objavljeno

0048-9697

1879-1026

10.1016/j.scitotenv.2019.06.118

Povezanost rada

Kemija, Biologija, Interdisciplinarne prirodne znanosti

Poveznice
Indeksiranost