engleski

THE VOLUNTEER STUDENTS’ KNOWLEDGE ABOUT THE IMPORTANCE OF PREANALYTICAL PHASE IN LABORATORY DIAGNOSTICS

Laboratory diagnostics consists of preanalytical (sampling, sample labeling, handling, storage, transportation and tests order), analytical (sample measurement) and postanalytical (results transmission) phases. Preanalytical phase is the most important for the sample quality and subsequently for the final laboratory results. The aim of the study was to assess the level of knowledge on preanalytical phase in group of students volunteering in Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb. The study was conducted by using anonymous online questionnaire with ten statements related to the preanalytical phase. The questionnaire was prepared with Google Forms and sent via email to students. Study included ten volunteers and eight had more than 8 correct answers. Results showed that students are aware of the importance of correct sample-labeling (100%) as well as proper choice of anticoagulant (90%). Students understand relevance of blood and anticoagulant ratio (100%) and handling the sample after sampling with anticoagulant (100%). Although they know that blood samples in tubes with red and yellow caps should not be mixed (100%), 60% are not aware that serum sample from tube with red cap should be separate immediately before storage. Similarly, volunteers know that hemolysis and lipemia are the most frequent interferences in serum (90%) but 50% of them do not know that hematuria interferes with total protein concentration in urine. Statements on storage (40%) and test ordering (40%) showed that volunteers do not have satisfying levels of knowledge. In conclusion, responses indicate that volunteers are aware of importance of preanalytical phase but their knowledge of all variables include in preanalytical processes should be improved. Results suggest the necessity of implementing preanalytical protocols in education programs in order to improve patient safety.

preanalytical phase ; volunteers ; questionnaire

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