engleski

Antifungal activity of the extracts of selected marine organisms

Marine organisms represent a rich source of bioactive compounds with a high potential in therapeutic and industrial applications. It has been proven that these compounds posses a wide range of bioactivities, such as antioxidant, antimicrobial, antiproliferative and anti- inflammatory. This study aimed to investigate antifungal activity of the extracts obtained with ultrasound-assisted extraction. Tested marine organisms included ascidian Aplydium conicum, green macroalga Codium bursa, brown macroalga Cystoseira sp. and cnidarian Eunicella cavolinii. Ultrasound-assisted extraction was performed at the ultrasound bath for each of mentioned organisms with two different solvents ; water was used for the extractions of polar compounds, while dimethylsulfoxide (DMSO) was used for non-polar compounds. The conditions during the extraction were the temperture of 30°C and the time of 30 minutes, the concentration in case of Codium bursa was 20 mL/g, while for the other organisms it was 10 mL/g. The extracts prepared in the water and DMSO were tested separately in order to compare their activities depending on the polarity of extracted compounds. The antifungal activity of these extracts was tested against the most common and problematic mycotoxigenic fungi including: Alternaria alternata, Aspergillus flavus, Aspergillus ochraceus, Fusarium gramniearum, Fusarium verticillioides and Penicillium expansum. Antifungal testing was performed with standardized microdilution method which is in accordance with the NCCLS guidelines, document M38-A. The obtained results showed that there is significant difference in the activity against tested fungal species depending on the used extract (water or DMSO).

Antifungal activity ; marine organisms

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