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Pregled bibliografske jedinice broj: 1003389

Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822)


Dragojević, Jelena
Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822), 2018., doktorska disertacija, Sveučilište u Osijeku, Osijek


CROSBI ID: 1003389 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822)

Autori
Dragojević, Jelena

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Sveučilište u Osijeku

Mjesto
Osijek

Datum
14.12

Godina
2018

Stranica
110

Mentor
Smital, Tvrtko

Ključne riječi
SLC transporters ; Danio rerio ; phylogenetic analysis ; synteny analysis ; tissue expression ; function ; ADME ; CRISPR/Cas9

Sažetak
Organic anion transporters (OATPs/OATs) are transmembrane proteins which belong to SLC21 and SLC22 subfamily of the SLC (Solute Carriers) family. They are responsible for the uptake of various endogenous and xenobiotic compounds into the cell. Despite their crucial role in toxicological response, their detailed ecotoxicological relevance and characteristics in non-mammalians are still unknown. Therefore, the main goal of this study was identification and molecular characterization of organic anion transporters in zebrafish (Danio rerio). Additional goal was the creation of zebrafish gene knockouts for the best characterized zebrafish transporter of organic anions, Oatp1d1. Seven organic anion transporters are present in zebrafish: Oat1, Oat2a, 2b, 2c, 2d, 2e and Oat3, encoded by genes slc22a6, slc22a7(a-e) and slc22a8, respectively. New model fluorescent substrates were identified for 5 zebrafish Oats, and high throughput in vitro assays for identification of interactors of these proteins were developed. Zebrafish Oats showed interaction with various endo- and xenobiotics. The first CRISPR/Cas9 zebrafish Oatp1d1 knockouts were succesfuly generated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-4806 - Identifikacija i funkcionalna karakterizacija (eko)toksikološki važnih polispecifičnih membranskih transportnih proteina u zebrici (Danio rerio) (Tvrtko Smital, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Sveučilište J. J. Strossmayera u Osijeku

Profili:

Avatar Url Jelena Dragojević (autor)

Avatar Url Tvrtko Smital (mentor)

Citiraj ovu publikaciju

Dragojević, Jelena
Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822), 2018., doktorska disertacija, Sveučilište u Osijeku, Osijek
Dragojević, J. (2018) 'Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822)', doktorska disertacija, Sveučilište u Osijeku, Osijek.
@phdthesis{phdthesis, author = {Dragojevi\'{c}, J.}, year = {2018}, pages = {110}, keywords = {SLC transporters, Danio rerio, phylogenetic analysis, synteny analysis, tissue expression, function, ADME, CRISPR/Cas9}, title = {Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822)}, keyword = {SLC transporters, Danio rerio, phylogenetic analysis, synteny analysis, tissue expression, function, ADME, CRISPR/Cas9}, publisherplace = {Osijek} }