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Pregled bibliografske jedinice broj: 1003308

Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors


Štimac, Adela; Tokić, Matea; Ljubetič Ajasja; Vuletić, Tomislav; Šekutor, Marina; Požar, Josip; Leko, Katarina; Hanževački, Marko; Frkanec Leo; Frkanec, Ruža
Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors // Organic & biomolecular chemistry, 17 (2019), 4640-4651 doi:10.1039/C9OB00488B (međunarodna recenzija, članak, znanstveni)


Naslov
Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors

Autori
Štimac, Adela ; Tokić, Matea ; Ljubetič Ajasja ; Vuletić, Tomislav ; Šekutor, Marina ; Požar, Josip ; Leko, Katarina ; Hanževački, Marko ; Frkanec Leo ; Frkanec, Ruža

Izvornik
Organic & biomolecular chemistry (1477-0520) 17 (2019); 4640-4651

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Adamantyl guanidine ; amphiphilic ß-cyclodextrin vesicles ; host-guest complexes ; liposomes ; nonviral gene delivery vectors ; supramolecular systems

Sažetak
Multicomponent self-assembled supramolecular nanovesicles based on an amphiphilic derivative of β-cyclodextrin and phosphatidylcholine liposomes (PC-liposomes) functionalized with four structurally different adamantyl guanidines were prepared and characterized. Incorporation efficiency of the examined adamantyl guanidines as well as size and surface charge of the prepared supramolecular nanovesicles was determined. Changes in the surface charge of the prepared nanovesicles confirmed that guanidinium groups were exposed on the surface. ITC and 1H NMR spectroscopy complemented by molecular dynamics (MD) simulations were used to elucidate the structural data and stability of the inclusion complexes of β-cyclodextrin and adamantyl guanidines (AG1–5). The results are consistent and point to a significant contribution of the guanylhydrazone residue to the complexation process for AG1 and AG2 with β-cyclodextrin. In order to evaluate the potential of the self- assembled supramolecular nanomaterial as a nonviral gene delivery vector, fluorescence correlation spectroscopy was used. It showed that the prepared nanovesicles functionalized with adamantyl guanidines AG1–4 effectively recognize and bind the fluorescently labelled DNA. Furthermore, gel electrophoretic assay confirmed the formation of nanoplexes of functionalized nanovesicles and plasmid DNA. These findings together suggest that the designed supramolecular nanovesicles could be successfully applied as nonviral gene delivery vectors.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekt / tema
HRZZ-IP-11-2013-7387
HRZZ-IP-11-2013-8238
HRZZ-IP-2014-09-7309 - razvoj supramolekulskih receptora kationa i aniona (Vladislav Tomišić, )
HRZZ-IP-2018-01-6910 - Sinteza supramolekulskih samo-udruženih nanostruktura za izgradnju naprednih funkcionalnih materijala (Leo Frkanec, )

Ustanove
Institut za fiziku, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Sveučilište u Zagrebu

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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