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Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors (CROSBI ID 265339)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Šekutor, Marina ; Požar, Josip ; Leko, Katarina ; Hanževački, Marko ; Frkanec, Leo ; Frkanec, Ruža Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors // Organic & biomolecular chemistry, 17 (2019), 4640-4651. doi: 10.1039/C9OB00488B

Podaci o odgovornosti

Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Šekutor, Marina ; Požar, Josip ; Leko, Katarina ; Hanževački, Marko ; Frkanec, Leo ; Frkanec, Ruža

engleski

Functional self-assembled nanovesicles based on β-cyclodextrin, liposomes and adamantyl guanidines as potential nonviral gene delivery vectors

Multicomponent self-assembled supramolecular nanovesicles based on an amphiphilic derivative of β-cyclodextrin and phosphatidylcholine liposomes (PC-liposomes) functionalized with four structurally different adamantyl guanidines were prepared and characterized. Incorporation efficiency of the examined adamantyl guanidines as well as size and surface charge of the prepared supramolecular nanovesicles was determined. Changes in the surface charge of the prepared nanovesicles confirmed that guanidinium groups were exposed on the surface. ITC and 1H NMR spectroscopy complemented by molecular dynamics (MD) simulations were used to elucidate the structural data and stability of the inclusion complexes of β-cyclodextrin and adamantyl guanidines (AG1–5). The results are consistent and point to a significant contribution of the guanylhydrazone residue to the complexation process for AG1 and AG2 with β-cyclodextrin. In order to evaluate the potential of the self- assembled supramolecular nanomaterial as a nonviral gene delivery vector, fluorescence correlation spectroscopy was used. It showed that the prepared nanovesicles functionalized with adamantyl guanidines AG1–4 effectively recognize and bind the fluorescently labelled DNA. Furthermore, gel electrophoretic assay confirmed the formation of nanoplexes of functionalized nanovesicles and plasmid DNA. These findings together suggest that the designed supramolecular nanovesicles could be successfully applied as nonviral gene delivery vectors.

adamantyl guanidine ; amphiphilic ß-cyclodextrin vesicles ; host-guest complexes ; liposomes ; nonviral gene delivery vectors ; supramolecular systems

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Podaci o izdanju

17

2019.

4640-4651

objavljeno

1477-0520

1477-0539

10.1039/C9OB00488B

Povezanost rada

Kemija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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