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Immunophenotyping of CD3+CD4+ and CD3+CD8+ peripheral blood lymphocytes in psoriasis vulgaris

Vulgar psoriasis (PV) is a common, relapsing erythematosquamous dermatosis mediated by various inflammatory cells. Previous reports have demonstrated an important role for susceptible genetic background, impaired skin barrier and uncontrolled immune response, particularly T cells, in disease etiology. Accordingly, increased numbers of CD3+CD4+ T cell helper (Th) and CD3+CD8+ cytotoxic (Tc) lymphocytes, particularly Th1, Th17, Tc1 and Tc17 cells, have been reported in psoriatic skin lesions. However, the specific shares and migration patterns of peripheral CD3+CD4+ and CD3+CD8+ lymphocyte populations in PV are still insufficiently clarified. Thus, we aim to investigate whether CD3+CD4+ and CD3+CD8+ T cell frequency and phenotype are affected in PV patients. To achieve this goal, peripheral blood mononuclear cells (PBMC) were isolated from 4 ml of heparinized blood samples collected from 14 healthy controls (CTRL) and 14 patients diagnosed with PV. In the following step, PBMCs were stained with the CD3 FITC, CD4 PE-Cy7 and CD8 PerCP monoclonal antibodies. Dead cells were excluded using LIVE/DEAD Fixable Near IR Kit. Flow cytometry was performed on BD FACS Canto II cytometer and data were analysed with the FloLogic software. Compared to controls, psoriatic patients had significantly lower frequency of circulating CD3+CD8+high T cells [PV vs CTRL (mean±SD): 33.3±9.2 vs 41.8±7.4, P=0.0083 (Man-Whitney test)], whereas peripheral CD3+CD4+ T cell count was significantly higher [PV vs CTRL: 59±9.9 vs 49.3±10.4, P=0.023]. Our preliminary findings support the role of peripheral T cell compartments in psoriasis and warrant functional characterisation of CD4+ and CD8+ T cell population in larger studies.

T-lymphocyte, flow cytometry, psoriasis vulgaris

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