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Pregled bibliografske jedinice broj: 1002884

DNA/RNA recognition controlled by the glycine linker and the guanidine moiety of phenanthridine peptides


Matić, Josipa; Šupljika, Filip; Tandarić, Tana; Dukši, Marko; Piotrowski, Patryciusz; Vianello, Robert; Brozovic, Anamaria; Piantanida, Ivo; Schmuck, Carsten; Radić Stojković, Marijana
DNA/RNA recognition controlled by the glycine linker and the guanidine moiety of phenanthridine peptides // International journal of biological macromolecules, 134 (2019), 422-434 doi:10.1016/j.ijbiomac.2019 (međunarodna recenzija, članak, znanstveni)


Naslov
DNA/RNA recognition controlled by the glycine linker and the guanidine moiety of phenanthridine peptides

Autori
Matić, Josipa ; Šupljika, Filip ; Tandarić, Tana ; Dukši, Marko ; Piotrowski, Patryciusz ; Vianello, Robert ; Brozovic, Anamaria ; Piantanida, Ivo ; Schmuck, Carsten ; Radić Stojković, Marijana

Izvornik
International journal of biological macromolecules (0141-8130) 134 (2019); 422-434

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA/RNA recognition ; Pyrrole guanidine peptide ; DNA/RNA chiral probe

Sažetak
The binding of four phenanthridine-guanidine peptides to DNA/RNA was evaluated via spectrophotometric/microcalorimetric methods and computations. The minor structural modifications-the type of the guanidine group (pyrrole guanidine (GCP) and arginine) and the linker length (presence or absence of glycine)- greatly affected the conformation of compounds and consequently the binding to double- (ds-) and single- stranded (ss-) polynucleotides. GCP peptide with shorter linker was able to distinguish between RNA (A-helix) and DNA (Bhelix) by different circular dichroism response at 295 nm and thus can be used as a chiral probe. Opposed to the dominant stretched conformation of GCP peptide with shorter linker, the more flexible and longer linker of its analogue enabled the molecule to adopt the intramolecularly stacked form which resulted in weaker yet selective binding to DNA. Beside efficient organization of ss-polynucleotide structures, GCP peptide with shorter linker bound stronger to ss-DNA/RNA compared to arginine peptides which emphasize the importance of GCP unit.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2014-09-3386 - Dizajn i sinteza novih dušikovih heterocikličkih fluorofora i fluorescentnih nanomaterijala kao kemijskih senzora za pH i metalne ione (Robert Vianello, )
HRZZ-IP-2018-01-4694 - Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima (Marijana Radić Stojković, )
HRZZ-IP-2018-01-5475 - Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina (Ivo Piantanida, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Fidelta d.o.o.

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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