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Pregled bibliografske jedinice broj: 1002540

Comparative Analysis and Validation of Different Steps in Glycomics Studies


Trbojević-Akmačić, I.; Ugrina, I.; Lauc, G.
Comparative Analysis and Validation of Different Steps in Glycomics Studies // Methods in Enzymology / Arun K., Shukla (ur.).
Netherlands: Elsevier, 2017. str. 37-55 doi:10.1016/bs.mie.2016.09.027


CROSBI ID: 1002540 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Comparative Analysis and Validation of Different Steps in Glycomics Studies

Autori
Trbojević-Akmačić, I. ; Ugrina, I. ; Lauc, G.

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Methods in Enzymology

Urednik/ci
Arun K., Shukla

Izdavač
Elsevier

Grad
Netherlands

Godina
2017

Raspon stranica
37-55

ISBN
9780121819613

ISSN
0076-6879

Ključne riječi
Glycans High throughput Glycome analysis HILIC UPLC Method validation

Sažetak
Large-scale glycomics studies enable identification of aberrant glycosylation patterns in disease and provide information about functional relevance of individual glycans through genome-wide association studies. Developed high-throughput methodologies have to be sensitive, robust, and stable during long periods of time (few months) to be able to reliably detect small biological variations in glycosylation. Here, we describe a simple, robust, and affordable protocol for immunoglobulin G N-glycan analysis by hydrophilic interaction liquid chromatography–ultra-performance liquid chromatography (HILIC-UPLC), as well as useful strategies for method optimization: Plackett–Burman screening design and analysis of source of variation. We put our focus on experimental design for high-throughput glycan analysis, critical steps in sample preparation procedure for obtaining high-quality data, and propose a validation protocol relevant for high-throughput methods in terms of their long-term robustness and ability to detect biologically relevant changes in glycosylation. The quality of the procedure was assessed by employing appropriate experimental designs and subsequent statistical techniques.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
EK-FP7-278535 (EK - FP7-HEALTH-2011-two-stage)
EK-FP7-315997 (EK - FP7-REGPOT-2012-2013-1)
EK-FP7-324400 (EK - FP7-PEOPLE-2012-IAPP)
EK-FP7-602736 (EK - FP7-HEALTH-2013-INNOVATION-1)

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.

Profili:

Avatar Url Gordan Lauc (autor)

Avatar Url Ivo Ugrina (autor)

Avatar Url Irena Trbojević Akmačić (autor)

Citiraj ovu publikaciju

Trbojević-Akmačić, I.; Ugrina, I.; Lauc, G.
Comparative Analysis and Validation of Different Steps in Glycomics Studies // Methods in Enzymology / Arun K., Shukla (ur.).
Netherlands: Elsevier, 2017. str. 37-55 doi:10.1016/bs.mie.2016.09.027
Trbojević-Akmačić, I., Ugrina, I. & Lauc, G. (2017) Comparative Analysis and Validation of Different Steps in Glycomics Studies. U: Arun K., S. (ur.) Methods in Enzymology. Netherlands, Elsevier, str. 37-55 doi:10.1016/bs.mie.2016.09.027.
@inbook{inbook, editor = {Arun K., S.}, year = {2017}, pages = {37-55}, DOI = {10.1016/bs.mie.2016.09.027}, keywords = {Glycans High throughput Glycome analysis HILIC UPLC Method validation}, doi = {10.1016/bs.mie.2016.09.027}, isbn = {9780121819613}, issn = {0076-6879}, title = {Comparative Analysis and Validation of Different Steps in Glycomics Studies}, keyword = {Glycans High throughput Glycome analysis HILIC UPLC Method validation}, publisher = {Elsevier}, publisherplace = {Netherlands} }

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