engleski

Pentadecapeptide BPC 157 cream improves burn-wound healing and attenuates burn-gastric lesions in mice.

The effects of the gastric pentadecapeptide BPC 157, administered topically or systemically in burned mice were investigated. This agent is known to have a beneficial effect in variety models of gastrointestinal lesions, as well as on wound or fracture healing, also when given locally. Deep partial skin thickness burns (1.5cmx1.5cm) covering 20% of total body area, were induced under anesthesia on the back of the mice by controlled burning and gastric lesions were assessed 1, 2, 3, 7, 14 and 21 days following injury. The first application was immediately following burning, and thereafter, once daily, last 24h before sacrifice. In the initial experiments, exposition to direct flame for 5 seconds, the BPC 157 was applied at 10 mg or 10 ng/kg b.w. i.p. by injection or alternatively, topically, at the burn, as a thin layer of cream (50 mg of BPC 157 dissolved in 2 ml of distilled water was mixed with 50 g of commercial neutral cream (used also as local vehicle-control)), while silver sulfadiazine 1% cream was a standard agent acting locally. Others received no local medication: they were treated i.p. by injection of distilled water (distilled water-control) or left without any medication (control). In subsequent experiments involving deeper burns (direct flame for 7 seconds), BPC 157 creams (50 ľg, 5 ľg, 500 ng, 50 ng or 5 ng of BPC 157 dissolved in 2 ml of distilled water was mixed with 50 g of commercial neutral cream), or vehicle as a thin layer of cream, were applied topically, at the burn. Compared with untreated controls, in both experiments, in the BPC 157 cream treated mice all parameters of burn healing were improved throughout the experiment: less edema was observed and inflammatory cells number was decreased, less necrosis, an increased number of capillaries along with an advanced formation of dermal reticulin and collagen fibers and an increased number of preserved follicles were observed. Two weeks after injury, BPC 157 cream-treated mice completely reversed the otherwise poor reepithelization ratio noted in the untreated control or mice treated with only cream vehicle. Tensiometry investigation showed an increased breaking strength and relative elongation of burned skin, while water content in burned skin decreased. This was, however, not the case with the vehicle cream or silver sulfadiazine cream-mice. Relative to the control values, in silver sulfadiazine cream-treated mice, only collagen fiber formation was increased, in addition to a decreased inflammatory cells number. Relative to control values, BPC 157 given intraperitoneally decreased the number of inflammatory cells, lowered water content in burned skin, and raised breaking strength and relative elongation of burned skin during tensiometry. Through the experimental period gastric lesions were continously noted in all thermally injured mice left without local medication and they were consistently attenuated only by BPC 157 treatments: either given intraperitoneally (at either dose), or given locally (at either concentrations). Other treatments (i.e., local treatment with silver sulfadiazine cream or neutral cream in mice subjected for 5 seconds to direct flame), lead to only poor, if any attenuation. This stable gastric pentadecapeptide appears to be active and give a stimulation to burn healing at the defect site. The agent may act by causing an upregulation of the growth factors, as well as influences other local factors.

pentadecapeptide BPC 157; cream; burn-wound healing; burn-gastric lesions; collagen/BMP-6; mice

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