engleski

Vibrio populations and preliminary assessment of health status of European seabass from two marine fish farms in eastern Adriatic Sea

Aquaculture is an important industrial sector for both Montenegro and Croatia. Marine fish culture in both countries is dominated by European sea bass (Dicentrarchus labrax). Development and appearance of diseases among cultured fish is the result of the interactions among host, pathogens and environment. Microbiological survey in the two marine fish farms with intensive aquaculture of European seabass was conducted in Montenegro and Croatia in the spring and autumn of 2017/2018. All bacterial isolates affecting the farmed fish during these two periods were investigated, with focus on the identification and antimicrobial resistance. The microbial counts were carried out on two media: heterotrophic bacterial count on the Marine agar plates and Vibrio counts on the Thiosulfate Citrate Bile Sucrose (TCBS) plates. Isolates from Marine and TCBS agar plates were identified using MALDI TOF MS, whereas antimicrobial resistance was analyzed using disc diffusion method on the Muller Hinton II agar. The highest percentage of isolates were the members of the genera Vibrio and Photobacterium. Finding of these opportunistic pathogens in geographically distinct fish farms is important regarding sea bass health status issue, particularly by the fact that resistant strains of these bacteria could be widespread via currents. The study reveals the presence of opportunistic bacterial pathogens as part of the bacterial community of farmed European seabass and surrounding marine water.

Vibrio ; Photobacterium ; MALDI TOF MS ; Montenegro ; Croatia ; Dicentrarchus labrax

Diversity of Vibrio spp. in Mediterranean mussel (Mytilus galoprovincialis) originating from aquaculture environments in Montenegro and Croatia