engleski

Risk factors and molecular predispositions for cervical dysplasia among women from east Croatia

high-risk Human papillomavirus (HR HPV) – induced cervical infection, HR HPV-related cervical dysplasia, HR HPV genotypes with two Toll-like receptor (TLR) 9 gene polymorphisms and other risk factors. Methods: During a three-year period, 100 women positive for cervical HR HPV infection (97 with cervical dysplasia and 3 positive women without dysplasia) were genotyped using the Linear Array HPV Genotyping assay (Roche Diagnostics). Furthermore, two polymorphisms of TLR9 (-1486T/C, rs187084 and 2848C/T rs352140) were determined using real-time Polymerase Chain Reaction ; 50 HR HPV negative women of similar ethnicity were included as controls. Results: This study showed that infections with HPV 16 in women with cervical dysplasia were more frequently found compared with HPV 18 infections (p=0.0539). Comparison between HR HPV positive and negative women showed significant association between age >35 years (p=0.0058), being unmarried women (p=0.0001), nocondom usage (p=0.0304) and active tobacco smoking (p=0.0376) with HR HPV cervical infection. No significant associations between two TLR9 gene polymorphisms, HR HPV infection and cervical dysplasia were found. Conclusion: Our results indicated that: i) women with cervical dysplasia showed significant higher rate of HR HPV 16 infection compared to HR HPV 18, ii) HR HPV – infection was strongly correlated with social risk factors and iii) TLR9 gene polymorphisms (rs187084 ; rs352140) did not correlate with HR HPV infection and cervical dysplasia. Further genome- wide association studies could open new frontier in understanding the relationship between polymorphisms at TLR9 and immunological mechanisms in HPV-induced carcinogenesis.

carcinogenesis ; human papillomavirus 16 ; human papilloma virus 18 ; toll-like receptor 9 ; uterine cervical dysplasia

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