engleski

Effect of low selenium diet on glutathione peroxidase 3 concentration in male Sprague-Dawley rats serum

AIM: Determination of antioxidative enzyme glutathione peroxidase 3 (GPx3) serum concentrations after consumption of food which contains different concentrations of selenium (Se). MATERIALS AND METHODS: Four weeks old Sprague- Dawley rats consumed food containing a different concentration of Se (food Divan) in its composition over a period of 10 weeks. Animals were divided in two groups: 1) normal Se (0.363 mg/kg Se) and 2) low Se (0.030 mg/kg Se). Each animal was weighed at the end of protocol, and serum samples were collected for determination of GPx3 concentrations. All experimental procedures were harmonized with ‘European Convention for the Protection of Vertebrate Animals and approved by the Ethics Committee of the Faculty of Medicine in Osijek and the Ministry of Agriculture of the Republic of Croatia. RESULTS: Different concentration of Se in food did not change body weight. Food which satisfies the amount of Se according to the guidelines of World Health Organization has significantly increased GPx3 enzyme concentration (13.96±0.42 mg/ml) when compared to low selective Se (12.04±0.33 mg/ml, p =0.002). CONCLUSION: Serum concentration of antioxidant enzyme GPx3 depends on the proportion of Se in food. Food containing a normal concentration of Se has been shown to be enriched with antioxidant GPx3 which, according to numerous studies, has a protective role in the human body, in comparison with food poor with Se.

antioxidative enzymes; glutathione peroxidase 3; Se; Sprague-Dawley rats

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