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Pregled bibliografske jedinice broj: 1000453

On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre


Reintjes, Greta; Tegetmeyer, Halina E.; Bürgisser, Miriam; Orlić, Sandi; Tews, Ivo; Zubkov, Mikhail; Voß, Daniela; Zielinski, Oliver; Quast, Christian; Glöckner, Frank Oliver et al.
On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre // Applied and environmental microbiology, 85 (2019), 14; 1-14 doi:10.1128/AEM.00184-19 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1000453 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre

Autori
Reintjes, Greta ; Tegetmeyer, Halina E. ; Bürgisser, Miriam ; Orlić, Sandi ; Tews, Ivo ; Zubkov, Mikhail ; Voß, Daniela ; Zielinski, Oliver ; Quast, Christian ; Glöckner, Frank Oliver ; Amann, Rudolf ; Ferdelman, Timothy G. ; Fuchs, Bernhard M.

Izvornik
Applied and environmental microbiology (0099-2240) 85 (2019), 14; 1-14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bacterial diversity ; NGS ; SPG

Sažetak
The South Pacific Gyre (SPG) covers 10% of the ocean's surface and is often regarded as a marine biological desert. To gain an on-site overview of the remote, ultra-oligotrophic microbial community of the SPG we developed a novel on-board analysis pipeline, which combines next-generation sequencing with fluorescence in situ hybridisation and automated cell enumeration. We tested the pipeline during the SO245 "UltraPac" cruise from Chile to New Zealand and found that the overall microbial community of the SPG was highly similar to that of other oceanic gyres. The SPG was dominated by 20 major bacterial clades, including SAR11, SAR116, AEGEAN-169 marine group, SAR86, Prochlorococcus, SAR324, SAR406, and SAR202. Most of the bacterial clades showed a strong vertical (20 m – 5000 m), but only a weak longitudinal (80°W – 160°W), distribution pattern. Surprisingly, in the central gyre Prochlorococcus, the dominant photosynthetic organism, had only low cellular abundances in the upper waters (20 - 80 m) and were more frequent around the 1% irradiance zone (100 - 150 m). Instead, the surface waters of the central gyre were dominated by SAR11, SAR86, and SAR116 clades known to harbour light-driven proton pumps. The alphaproteobacterial AEGEAN-169 marine-group was particularly abundant in the surface waters of the central gyre indicating a potentially interesting adaptation to ultraoligotrophic waters and high solar irradiance. In the future, the newly developed community analysis pipeline will allow for on-site insights into a microbial community within 35 hours of sampling, which will permit more targeted sampling efforts and hypothesis-driven research.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Sandi Orlić (autor)

Citiraj ovu publikaciju

Reintjes, Greta; Tegetmeyer, Halina E.; Bürgisser, Miriam; Orlić, Sandi; Tews, Ivo; Zubkov, Mikhail; Voß, Daniela; Zielinski, Oliver; Quast, Christian; Glöckner, Frank Oliver et al.
On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre // Applied and environmental microbiology, 85 (2019), 14; 1-14 doi:10.1128/AEM.00184-19 (međunarodna recenzija, članak, znanstveni)
Reintjes, G., Tegetmeyer, H., Bürgisser, M., Orlić, S., Tews, I., Zubkov, M., Voß, D., Zielinski, O., Quast, C. & Glöckner, F. (2019) On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre. Applied and environmental microbiology, 85 (14), 1-14 doi:10.1128/AEM.00184-19.
@article{article, year = {2019}, pages = {1-14}, DOI = {10.1128/AEM.00184-19}, keywords = {bacterial diversity, NGS, SPG}, journal = {Applied and environmental microbiology}, doi = {10.1128/AEM.00184-19}, volume = {85}, number = {14}, issn = {0099-2240}, title = {On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre}, keyword = {bacterial diversity, NGS, SPG} }
@article{article, year = {2019}, pages = {1-14}, DOI = {10.1128/AEM.00184-19}, keywords = {bacterial diversity, NGS, SPG}, journal = {Applied and environmental microbiology}, doi = {10.1128/AEM.00184-19}, volume = {85}, number = {14}, issn = {0099-2240}, title = {On site analysis of bacterial communities of the ultra-oligotrophic South Pacific Gyre}, keyword = {bacterial diversity, NGS, SPG} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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